Journal: Cardiology Research

Article Title: Nuclear Respiratory Factor-1 Ameliorates Heart Failure by Suppressing Cardiomyocyte Pyroptosis-Associated Signaling Via the Downregulation of Gasdermin D and Caspase-1

doi: 10.14740/cr2153

Figure Lengend Snippet: NRF-1 is negatively correlated with pyroptosis in HF patients. (a) Representative images of echocardiography in HF patients and NF controls. (b–g) Serum NT-proBNP, IL-18, IL-1β, NRF-1, GSDMD and caspase-1 level in HF patients (n = 15) and NF controls (n = 10). *P < 0.05, **P < 0.01, ***P < 0.001. GSDMD: gasdermin D; HF: heart failure; IL: interleukin; NF: normal cardiac function; NRF-1: nuclear respiratory factor-1; NT-proBNP: N-terminal pro-B-type natriuretic peptide.

Article Snippet: ELISA kits were used to detect N-terminal pro-B-type natriuretic peptide (NT-proBNP, EK1393, Multi Science, Hangzhou, China), NRF-1 (E15022h, EIAab, Wuhan, China), IL-18 (EK118S, Multi Science, Hangzhou, China), IL-1β (EK101B, Multi Science, Hangzhou, China), gasdermin D (GSDMD) (E15480h, EIAab, Wuhan, China), and caspase-1 (E1592h, EIAab, Wuhan, China), following the instructions of manufacturers.

Techniques:

Journal: Frontiers in Cardiovascular Medicine

Article Title: A swine model of severe chronic thromboembolic pulmonary hypertension induced by repeated pulmonary artery long suture injection

doi: 10.3389/fcvm.2025.1736958

Figure Lengend Snippet: Right ventricular myocardium gene expression (A) and protein levels (B) , and circulating plasma mediator levels (C) in chronic thromboembolic pulmonary hypertension (CTEPH) and sham (sham). Gene expression data depicts genes involved in myofilament remodeling (MYH6 and MYH7, α- and β-myosin heavy chain isoforms, respectively), extracellular matrix fibrosis (COL1A1 and COL3A1, type I and III collagen chains, respectively), Ca 2+ -handling (ATP2A2, sarcoendoplasmic reticulum calcium ATPase 2; RYR2, cardiac ryanodine receptor), and neurohumoral mediators (NPPB, B-type natriuretic peptide; EDN1, endothelin-1; TNF, tumor necrosis factor-α). Data were averaged upon normalization for 2 internal control genes, GAPDH (glyceraldehyde-3-phosphate dehydrogenase) and RPL4 (ribosomal protein L4). Calcium-handling protein levels of sarcoendoplasmic reticulum Ca2+-ATPase 2a (SERCA2a) normalized for GAPDH and phospho-phospholamban (PLB) normalized for total PLB and representative Western blot bands are presented in (B) , whilst mediator plasma levels of N-terminal pro–B-type natriuretic peptide (NT-proBNP) and endothelin-1 (ET-1) are presented in panel C. In panels A and B data are presented relative to Sham reference levels (dashed lines). * P < 0.05 vs. Sham by Student's t -test or Mann–Whitney U -test, according to assumptions ( n = 6 and 7 in Sham and CTEPH, respectively).

Article Snippet: Previously aliquoted plasma samples were thawed on ice and assayed for N-terminal pro–B-type natriuretic peptide (NT-proBNP; CSB-EQ027465PI, Cusabio) and endothelin-1 (ET-1; ADI-900-020A, Enzo Life Sciences Inc) in duplicate according to the manufacturer's instructions.

Techniques: Gene Expression, Clinical Proteomics, Control, Western Blot, MANN-WHITNEY

Journal: RSC Advances

Article Title: Yixinshu ameliorates hippocampus abnormality induced by heart failureviathe PPARγ signaling pathway

doi: 10.1039/c7ra10650e

Figure Lengend Snippet: Fig. 1 YXS improves the cardiac function of rats with heart failure. (A, B) Averaged data left ventricular ejection fraction (LVEF, A) and left ventricular fractional shortening (LVFS, B) assessed by echocardiography in rats. (C, D) Effects of YXS on BNP and NT-proBNP determined via ELISA assay. The plasma level of heart failure-related bio-marker including BNP (C), and NT-proBNP (D) were determined by ELISA assay in each group of rats vs. the model group, **P < 0.01; vs. the sham group, #P < 0.05, n ¼ 4–6 rats per group.

Article Snippet: Commercial ELISA test kits were used in the assessment of the hippocampal samples and serum: rat IL1-beta ELISA kit (eBioscience, USA), rat TNF-alpha ELISA Kit (eBioscience, USA), rat IL-6 ELISA kit (eBioscience, USA), rat BNP ELISA kit (Novus, Hong Kong) and NT-proBNP ELISA kit (Novus, Hong Kong).

Techniques: Enzyme-linked Immunosorbent Assay, Clinical Proteomics, Marker

Journal: RSC Advances

Article Title: Yixinshu ameliorates hippocampus abnormality induced by heart failureviathe PPARγ signaling pathway

doi: 10.1039/c7ra10650e

Figure Lengend Snippet: Fig. 2 YXS protects the hippocampus in rats with heart failure. (A) Expression of the tight junction proteins ZO-1 in the hippocampi of rats. Original Western blots shown on the upper panels. b-Actin was used as a loading control. Bar graphs are gray values. (B) Expression of the proteins AQP4 in the hippocampus of rats. Original Western blots shown on the upper panels. b-Actin was used as a loading control. Bar graphs are gray values. (C–E) The levels of pro-inflammatory cytokines, IL1-beta, TNF-alpha and IL-6, were detected by ELISA. Data are mean SD. vs. model, *p < 0.05, ***<0.001; vs. sham, #P < 0.05. n ¼ 4–6 animals per group.

Article Snippet: Commercial ELISA test kits were used in the assessment of the hippocampal samples and serum: rat IL1-beta ELISA kit (eBioscience, USA), rat TNF-alpha ELISA Kit (eBioscience, USA), rat IL-6 ELISA kit (eBioscience, USA), rat BNP ELISA kit (Novus, Hong Kong) and NT-proBNP ELISA kit (Novus, Hong Kong).

Techniques: Expressing, Western Blot, Control, Enzyme-linked Immunosorbent Assay

Journal: ESC Heart Failure

Article Title: Multiorgan characterization of inflammasome component expression in a rat model of advanced heart failure

doi: 10.1002/ehf2.15362

Figure Lengend Snippet: Characterization of the TAC model. Upper panel: morphology and clinical parameters of heart failure. (A) Representative M‐mode echocardiographic images at the midpapillary muscle level. (B) Left ventricular mass (LV mass) calculated from echocardiographic data. (C) Ejection fraction (EF) calculated from invasive P‐V measurement data. (D) Plasma N‐terminal prohormone of brain natriuretic peptide (NT‐proBNP) levels measured with ELISA. Lower panel: left ventricular haemodynamic parameters from invasive P‐V measurements. (E) Representative original steady‐state recordings (top) and loops obtained at different preloads during transient vena cava occlusions (bottom). (F) Systolic parameters—arterial elastance (E a ), slope of end‐systolic pressure‐volume relationship (ESPVR) and ventriculo‐arterial coupling (VAC). (G) Diastolic parameters—left ventricular end‐diastolic pressure (LVEDP), slope of end‐diastolic pressure‐volume relationship (EDPVR) and time constant of left ventricular pressure decay (Tau). Values are means ± SE. Normal distribution of the data was assessed with Shapiro–Wilk test. One‐way analysis of variance (ANOVA) followed by Tukey's post hoc test or Kruskal–Wallis test followed by Dunn's post hoc test were carried out depending on the distribution of the datasets. n = 6 (except for the ESPVR, VAC and EDPVR parameters in the TAC‐HF group, where n = 5 due to technical reasons). * P < 0.05 vs. sham; # P < 0.05 vs. TAC‐M. ELISA, enzyme‐linked immunosorbent assay; HF, heart failure; LVAWT d , left ventricular anterior wall thickness in diastole; LVEDD, left ventricular end‐diastolic diameter; LVPWT d , left ventricular posterior wall thickness in diastole; TAC, transverse aortic constriction.

Article Snippet: Plasma N‐terminal prohormone of brain natriuretic peptide (NT‐proBNP) concentration was assessed using rat N‐terminal pro‐brain natriuretic peptide, NT‐proBNP ELISA KIT (#CSB‐E08752r; Cusabio Technology, Houston, TX, USA) according to the manufacturer's protocol.

Techniques: Clinical Proteomics, Enzyme-linked Immunosorbent Assay